Day 2 :
Keynote Forum
Pierre Weiss
University of Nantes, France
Keynote: Hydrogels in regenerative dental medicine
Biography:
Pierre Weiss, PhD, DDS, received his Dental Doctorate in 1989. He pursued his Master of Science in Biomaterial (Nantes) in 1993, his PhD in Biomaterial (Nantes) in 1997. He is a Professor in Biomaterials of the University of Nantes in Dental Surgery Department and Hospital. He is the Head of REGOS team in UMRS 1229 RMeS Unit. His scientifi c activities are skeletal tissue engineering, physiochemistry in hydrophilic polymer to make hydrogels for synthetic extra cellular matrix, bone substitutes. His research interests include the chemistry and characterization of macromolecular solution and hydrogels to prepare synthetic extracellular matrices for tissue engineering of bone. He is also the President of the society for biohydrogels and the Vice Dean of the Nantes Dental school. He has more than 150 ISI indexed publications, 7 patents and Hirsh index: 41, 4000 citations.
Abstract:
This keynote will give an overview of hydrogels use for regenerative dental medicine. Hydrogels are three dimensional networks that have the ability to retain large amounts of water. More than 90% of the body is composed of macromolecules physically or chemically crosslinked in high water content and soluble compounds. Hydrogels have structural similarities with extracellular matrices (ECMs) and versatility that make them the ideal candidates in tissue engineering, drug delivery systems, and specifi c medical devices. 20 years ago, we designed injectable bioactive suspensions in water of calcium phosphate ceramics for bone and periapical regenerations. Because of leakage of these suspensions, we focused on injectable hydrogels before to set in situ by physical or chemical crosslinking to form 3D scaff olds. Finally, we set up a platform to develop a series of innovative hydrogels for bone, periodontal and endodontic tissue regeneration. We based our strategy on polysaccharides macromolecules because they are renewable materials, that originate from biological sources and generally are biocompatible, non-toxic and biodegradable. We developed a family of silanized and methacrylated macromolecules able to react forming biocompatible hydrogels. Th e silanized polymer are self-setting hydrogel able to covalently crosslink under pH variation, without addition of toxic crosslinking agent. Th e methacrylated polymers allow the in-situ photo crosslinking under standard dentistry lamp using cytocompatible photoinitiators. All these macromolecules could be combine in multicomponent hydrogels, representing a strategy for improving mechanical properties of biomaterials or to tailor particular properties to meet specifi c needs. For mineral scaff olding, we realized composites of calcium phosphates particles or cements with hydrogel, increasing the ductility and creating macroporous scaff old to propose foam bone cements. For endodontic applications we started to explore crushed dentine in high pH hydrogel for local autologous growth factors release and to investigate specifi c galenic formulation for dual molecules delivering. Future perspectives of our strategy are moving towards 3D printing and bio printing techniques. We will use our hydrogel platform to prepare tunable (bio) inks well adapted for specifi c applications in personalized dental medicine.
- Oral and Maxillofacial Surgery | Oral Health | Dental Hygiene | Orthodontics | Periodontics | Endodontics
Location: Olimpica 3+4
Chair
Ben F. Warner
The University of Texas Health Science Center at Houston, USA
Co-Chair
Pierre Weiss
University of Nantes, France
Session Introduction
Pauline Marie Chichiricco
University of Nantes, France
Title: Photo-crosslinkable membrane for guided periodontal tissue regeneration
Biography:
Pauline Marie Chichiricco obtained the Degree of Doctor in Pharmacy at Sapienza University in Rome, Italy. She is in her ultimate year PhD in a European program (Nanofar) between the Regenerative Medicine and Skeleton laboratory in Nantes, under the supervision of Professor Pierre Weiss and Doctor Xavier Struillou, and the Center for Education and Research on Macromolecules (CERM) at Liège University under the supervision of Professor Christine Jerome. Her research interest lies in biomaterials.
Abstract:
Periodontitis is an inflammatory disease resulting from the presence of oral bacteria biofi lm in periodontal tissue, which destroys the tooth-supporting attachment apparatus. Untreated inflammation can spread to all periodontal tissue and lead, ultimately, to tooth loss. Guided tissue regeneration (GTR) is a technique based on a barrier membrane designed to prevent colonization of the wound space by gingival cells. Indeed, these cells, with a faster migration and proliferation rate compared to those of bone and periodontal ligament ones, could interfere with the regeneration process. We developed a photo-crosslinkable formulation composed on methacrylated carboxymethyl Chitosan (MA-CMCS) and silanized hydroxypropylmethyl cellulose (Si-HPMC), that can be applied in situ and cured with a visible light lamp (λ 420-480 nm), already used in dentistry. Photocuring, in fact, appears the more appropriate technique for this application for its both shape and curing time control. We assessed the chemical and physico-chemical profile of this material, showing a quick time to obtain a solid membrane. To evaluate the degradation, the crosslinked material was soaked in highly concentrated lysozyme buffer at 37°C. Si-HMPC/ MA-CMCS material revealed a degradation profile between the two crosslinked biomaterials of reference. The in vitro biocompatibility was demonstrated by Neutral red assay using murine fibroblast (L929). Moreover, we studied the barrier eff ect of using primary cells, human gingival fi broblasts, observing the cells by confocal microscopy. No cells invasion was observed in Si-HMPC/MA-CMCS material. In addition, we set up an ex vivo experiment culturing human gingival explants in presence of our biomaterial and using pullulan porous material as a negative control. After culture, the samples were stained with hematoxylin, eosin Y and safranin (HES). The histological sections revealed that the biomaterial exhibited the barrier eff ect in contact with soft tissue. In the next future, in vivo experiment will be performed to further characterize the material for periodontal regeneration.
Akram Belmehdi
Mohammed V University, Kingdom of Morocco
Title: Successful conservative management of oral lesions by decompression or marsupialization procedure: A case series
Biography:
Akram Belmehdi is a Dental Doctor and a Specialist in the Department of Oral Surgery, Faculty of Dentistry, Mohamed V University, Rabat, Morocco. He is the Member of the Research Committee of Oral Biology and Pathology at the same university. He has published 6 papers in reputed journals and has been serving as a Reviewer of Journal of Medical and Surgical Research. He has participated in more than 30 oral presentations and posters in national and international dental and medical conferences.
Abstract:
Introduction: Several treatments have been described for the management of oral cysts, although none has been accepted globally. Nowadays, decompression, marsupialization, enucleation and resection of lesions are accepted as valid. The benefits of marsupialization and decompression include the gradually decreasing of the cystic cavity; preserving the adjacent oral tissues, maintaining pulp vitality, avoiding dental extractions, preventing iatrogenic damage to adjacent noble structures, avoiding mandibular fractures and reducing the risk of recurrence. In all cases, a second surgery is needed to eliminate totally the cystic lesion after decompression. Decompression and marsupialization are procedures that require patient’s commitment. They need several control appointments and constant hygiene with repeated irrigation, gauze soaked in iodine of the cystic cavity.
Aim: The aim of this work is to evaluate the effectiveness of the decompression and marsupialization as the primary treatment of the cystic lesions of the jaws and oral mucosa and their reduction rates involving different factors.
Materials & Methodology: A total of 12 patients with cystic lesion of the jaw and mucus-containing cystic lesion of the minor salivary gland were treated with decompression and marsupialization followed or not by enucleation. Pre- and postdecompression clinical features and panoramic radiographs were analyzed.
Conclusions/Clinical significance: Decompression and marsupialization proved to be simple, low cost, relatively noninvasive, painless, and low recurrence techniques to treat oral cystic lesions. All patients treated with decompression were reported to have shown a reduction in lesion size and higher sensitivity with a larger lesion size. There was no diff erence in the eff ect of decompression based on age, and only one patient experienced recurrence of the cyst.
Biography:
Prathibha Prasad is a Dentist specialized in Oral Pathology working in Faculty of Dentistry, Gulf Medical University, United Arab Emirates. She is actively involved in the teaching and supervision of Dental students for Ten years. She has several publications in reputed peer-reviewed journals and happen to serve as Reviewer and Editor in many journals.
Abstract:
Cigarettes are not the only form of tobacco smoked; in fact, waterpipe has been used for smoking tobacco for centuries. The name Waterpipe tobacco smoking (WTS) is used for the last 2 decades in the English language scientifi c literature to refer to any variety of instruments that involves tobacco smoke passing through water before inhalation. The use of water pipe is increasing daily, in all age and sex groups. There is a dire need to study and document health eff ects of water pipe smoking in general and specifi cally on the oral cavity as it has not been done anywhere else in the world till date. Since the oral mucosa is exposed to the smoke and its toxins from WTS directly, it will defi nitely help in studying the eff ects on it at a cellular and genetic level. The general public perception is that WTS is harmless. The waterpipe used just as other forms of tobacco use can be regulated if there is more awareness of its health eff ects. We collected socio-demographical, behavioral data pertaining to WTS and cigarette smoking in the region and studied their toxicological eff ects on the oral mucosa.
Eynar Berdeli
Izmir Katip Celebi University, Turkey
Title: The use of pink composite in periodontal splinting: Pink-splinting
Biography:
Eynar Berdeli has completed his DDS from Ege University in 2016. He is a PhD student in the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Izmir Katip Celebi University in Turkey since 2017. He has also been an Observer in Forsyth Institute, Harvard University; and School of Dentistry, University of Louisville in 2015. His research interest includes: esthetics, oral biology, guided bone regeneration, aggressive periodontitis.
Abstract:
Splinting is a common treatment approach in dental practice to obtain stability and eliminate mobility. It has also been shown that splinting can decrease pain and discomfort during biting and chewing. In addition, splinting of the mobile tooth is recommended before regenerative periodontal treatment to obtain maximum benefits from the treatment. It was our experience that SRP instrumentation was made easier by the splinting due to the prevention of tooth mobility. As another benefit, the patient was no longer complaining about chewing or biting after splinting. With the aid of pink-splinting clinicians will enhance the esthetic results after periodontal treatment in patients with periodontal disease. In addition, they will also manage the papilla loss and its effects in patients. This technique may be life-saving in patients with aggressive periodontitis.
- Workshop
Location: Olimpica 3+4
Session Introduction
Ben F. Warner
The University of Texas Health Science Center at Houston, USA
Title: New technology that sees oral cancer before you do
Biography:
Ben F Warner received BA Biology from University of St. Thomas in Houston, MS Neurophysiology from University of Houston, DDS from University of Texas School of Dentistry, and MD from McGovern Medical School. He is a Clinical Associate Professor and Director of Diagnosis and Treatment Planning Clinic in the Department of General Practice and Dental Public Health at University of Texas Health Science Center at Houston, USA. He completed residencies, serving as Chief Resident, in Pathology and Laboratory Medicine at Texas Medical Center, and Oral Maxillofacial Head and Neck Fellowship at MD Anderson Cancer Center in the Department of Pathology. He is a Master of Academy of General Dentistry, and Fellow of International College of Dentists, American College of Dentists and Academy of Laser Dentistry. He is a Recipient of variety of research awards, and on several professional committees, journal editorial boards and charitable organizations.
Abstract:
New technology is now available to healthcare providers to aid in the visualization of oral lesions. Fortunately, this technology can lead to earlier cancer detection and improved prognosis. This conference workshop addresses the following: familiarized attendees with autofluorescence technology, demonstrate how to use these instruments, explain the differences in the available devices, show how autofluorescence varies with the area of the mouth, explain how to recognize mucosal changes and distinguish oral cancer from entities such as amalgam tattoo, bacteria, varicosities, physiologic pigmentation, and inflammation, help attendees develop a one minute oral examination protocol utilizing this new technology immediately upon returning to their office/clinic.