Kuenga Zam
Mahidol University, Thailand
Title: Adhesion of Candida albicans to vanillin incorporated self-curing orthodontic PMMA resin
Biography
Biography: Kuenga Zam
Abstract
Background: It has been observed that there is an increase in Candida carriers during the treatment with orthodontic removable appliance. Vanillin is a flavouring agent, which is known to have antioxidant and antimicrobial properties.
Objectives: The purpose of this study was to investigate the effect of vanillin incorporated PMMA on adhesion of Candida albicans.
Material & Methods: A total of 54 orthodontic self-curing PMMA resin samples were fabricated. The samples were divided into 3 groups depending on percentage of vanillin incorporated (0.1%, 0.5% and 0% as control). PMMA samples were coated with saliva. The adhesion assay was performed with C. albicans (ATCC 10231) and a clinical strain isolated from oral lesion of a patient. The adherent yeast cells were stained with crystal violet and counted under microscope by random selection of 3 fields at 10X magnification. The statistical significance was analysed by Kruskal-Wallis and Mann-Whitney non-parametric test.
Results: It was found that the PMMA resin samples with vanillin incorporation significantly reduced the adhesion of C. albicans as compared to the control group.
Conclusions: This study indicates that vanillin incorporated resin can impede the adhesion of C. albicans (ATCC 10231) about 45-56% and clinical strain about 55 to 60%. With further testing and development, vanillin can be employed as an antifungal agent to prevent adhesion of C. albicans to orthodontic self-curing PMMA resin.