24th International Conference on Dentistry and Dental Materials
Ain Shams University & Alazhar University, Egypt
Title: Effect of diode laser and photoactivated disinfection on root canal Enterococcus faecalis biofilm
Biography: Eman Maged Fouad Ahmed
Background: Over years, the main goal of endodontic therapy has focused on the total elimination or at least major reduction of root canal microorganisms, which has never been guaranteed yet. Recently photoactivated disinfection (PAD) through activation by laser irradiation has been suggested as a promising supplement to root canal disinfection. It is further enhanced by incorporation of concept of nanotechnology.
Aim: Aim of the study was to evaluate the antibacterial effect of PAD protocol with or without silver nanoparticles against E. faecalis biofilm.
Materials & Methods: 60 single rooted teeth were decoronated and the root canals were mechanically prepared. Then roots were inoculated with E. faecalis isolate and incubated at 37°C for 7 days. They were divided equally into three experimental groups and one positive control group. The first group canals were irrigated with 2.6%NaOCL for 5 minutes. Second group subjected to PAD protocol where canals were filled with 100µg/ml methylene blue (MB) and irradiated with 660 nm, diode laser at power of 250 mW for 180 seconds. Third group canals were inoculated with both NgNPs and MB and irradiated at same settings.
Results: They were assessed quantitively through counting the colony forming units
(CFU/ml) while scanning electron micrography was performed to address topographical changes of experimental groups. All of experimental groups resulted in significant high reduction in bacterial population, however the synergistic effect of NgNPS was very obvious and the colony counts reached 0. Micrographs confirmed the presence of the dense heterogeneous smear layer in G1, modification of smear layer with partial opening of dentinal tubules in G2 while demonstrated total absence of smear layer and clear surface of dentine in G3.
Conclusion: Incorporation of NaNPs with PAD protocol represents a very promising modality in regard to root canal disinfection.